A modified BioBrickââ??¢ assembly method was developed with higher fidelity than current protocols. The method\r\nutilizes a PCR reaction with a standard primer set to amplify the inserted part. Background colonies are reduced by\r\na combination of dephosphorylation and digestion with DpnI restriction endonuclease to reduce vector and insert\r\nbackground respectively. The molar ratio of the insert to vector in the ligation was also optimized, with the\r\naccuracy of the transformed construct approaching 100%.
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